Design of cocktail peptide vaccine against Cytomegalovirus infection

نویسندگان

  • Arezoo Esmaili Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran
  • Baratali Mashkani Department of Medical Biochemistry, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
  • Reza Karimi Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran
  • Saeid Amel Jamehdar Antimicrobial Resistance Research Center, Avicenna Research Institute, Mashhad University of Medical Science, Mashhad, Iran
  • Samira Tabaei Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran
چکیده مقاله:

Objective(s):Human Cytomegalovirus (HCMV) remains a major morbidity and mortality cause in immuno suppressed patients. Therefore, significant effort has been made towards the development of a vaccine. In this study, the expression of the pp65 and gB fusion peptides and Fc domain of mouse IgG2a as a novel delivery system for selective uptake of antigens by antigen-presenting cells (APCs) in Pichia pastoris yeast system were studied. Materials and Method: In this study, four immune dominant sequences in pp65 protein and 3 immuno dominant sequences in gB protein were selected according to literature review. Peptide linker -GGGGS- was used for construction of fusion peptide. This fusion peptide was cloned in the pPICZαA expression vector and transfected into P. pastoris host cells. Results: Dot blot and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) techniques showed that a high level of pp65-gB-Fc fusion peptide was expressed. Conclusion: This CMV pp65-gB-Fc fusion peptide could be a promising candidate for the development of a novel peptide vaccine.

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design of cocktail peptide vaccine against cytomegalovirus infection

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عنوان ژورنال

دوره 19  شماره 4

صفحات  449- 454

تاریخ انتشار 2016-04-01

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